DNA Vectors
Sino Biological pCMV3-C-GFPSpark Control Vector (C-terminal GFPSpark-tagged)
Control for the pCMV3-C-GFPSpark clone.
IBA LifeSciences pASK-IBA5C
pASK-IBA5C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), and the inducible tetracycline promoter/operator for the regulated expression of proteins.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. The recombinant protein will be localized in the cytoplasm.