DNA Vectors
Sigma Aldrich Fine Chemicals Biosciences CRISPR LacZ Positive Contr1EA
Recent publications using CRISPR/Cas9-mediated recombineering in E. coli tout editing efficiencies near 100% making CRISPR/Cas9-mediated recombineering the most powerful bacterial genome engineering method to date. In addition Cas9-mediated recombineering overcomes the dependence on a second recombination step avoids the creation of destabilizing scar sites can be used in multiplexing and is less time-consuming than previous protocols.Here we present a novel dual-vector CRISPR/Cas-mediated»-Red system for improved recombineering in E. coli. Our system is shown to facilitate homology-directed repair of DSBs created by Cas9 endonuclease enabling genetic alterations through chromosomal integration of a donor DNA.This plasmid is to be used in combination with the Cas9 Lambda Red homologous recombination plasmid for E. coli (CAS9BAC1P) as the positive control for your custom gene editing experiment. The custom gRNA (CRISPRBACD) can be designed and ordered through https//www.sigmaald
Agilent Technologies PADEASY-1 VECTOR
The pAdEasy-1 vector is a 33.4 kb plasmid containing most of the human adenovirus serotype 5 (Ad5) genome, with deletions in the genes E1 and E3. This vector has been developed to infect but not replicate in non-permissive target cells
Agilent Technologies PSHUTTLE-CMV VECTOR
pShuttle-CMV Vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb).
Agilent Technologies ADEASY ADENOVIRAL VECTOR SYSA
AdEasy Adenoviral Vector System employes Homologous recombination in E.coli. It has High-level protein expression. Infects both dividing and non-dividing cells with 100% transduction efficiency. Vitality hrGFP reporter is available.
Eurofins Discoverx pCMV- ProLink 2 Vector, 1 vial, 10 µg. (93-0171)
The pCMV- ProLink 2 Vector is a mammalian cloning vector for use in expressing a protein of interest fused to the ProLink 2 (PK 2) tag at the C-terminus. The PK tag is a version of ED used for EFC assays (in combination with EA). The ProLink 2 tag has a higher affinity for EA, making it ideal for use with weaker protein:protein interactions. Provided as a vial of 10 μg vector in 20 μL liquid buffer. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/reagent/vector/pcmv-2-vector-93-0171.
Eurofins Discoverx pCMV-ProLabel C Vector, 1 vial, 10 µg. (93-0012)
The pCMV-ProLabel C Vector is a mammalian cloning vector for use in expressing a protein of interest fused to the ProLabel (ProLabel) tag at the C-terminus. The ProLabel tag is a version of ED used for EFC assays (in combination with EA) where ED and EA are contained in separate cellular compartments. Provided as a vial of 10 μg vector in 20 μL liquid buffer. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/reagent/vector/pcmv-c-vector-93-0012.
Teknova AAV•Tek™ AEX Buffer: AAV2 - Elution Buffer 1B
This product is proprietary to Teknova. Please get in touch if you'd like more information or have any questions.
Diagnostic Biosystems CMV COCKTAIL Cytomegalovirus (CMV Cocktail)
This cocktail antibody stains infected cells giving a nuclear staining pattern with an immediate early and an early CMV antigen.
AB Vector LLC ProGreen™ | 25uL
ProGreen™ baculovirus genomic vector DNA encodes Aequorea victoria green-fluorescent protein (GFP) Fig. 1. GFP allows monitoring of recombinant virus propagation, optimization of cultivation conditions, and titration of virus stocks by "terminal green dilution." The polyhedrin site is reserved for insertion of target genes under control of strong polyhedrin promoter. Initially, the polyhedrin site was occupied by E.coli β-galactosidase gene. To allow insertion of a target gene, β-galactosidase gene was removed from the baculovirus DNA using Bsu36.I restriction endonuclease which cleaves immediately downstream of the polyhedrin promoter, in the β-galactosidase gene and in the downstream essential gene ORF 1629.
System Biosciences LLC PCDH-CMV-MCS-EF1A-PURO CDNA DU
pCDH-CMV-MCS-EF1α-Puro cDNA Dual Promoter Cloning and Expression Lentivector
Clontech Labs 3P T-Vector pMD™19 (Simple), 1ug
T-Vector pMD19 (Simple) is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the cloning site improve the efficiency of ligation of PCR products which contain A-overhangs at 3' -ends. The multiple cloning sites in the lac Z gene sequence have been deleted from this vector, but its β-galactosidase activity is not disrupted. Therefore, transformant colonies containing recombinant plasmids can be identified by blue/white screening. Due to lack of multiple cloning sites in the vector, the inserted PCR product cannot be recut using restriction enzymes. When the insert DNA fragment is recut from the vector, the PCR primers should be used to add a restriction site to the 5'-end for restriction enzyme digestion and subcloning. T-Vector pMD19 (Simple) is supplied with a Control Insert (500 bp) DNA for positive control reactions.
AB Vector LLC GC (Green Control), 1 mL
Positive control baculovirus in experiments where GFP fluorescence is used to monitor virus propagation, optimize cultivation conditions, or titrate of virus stocks.
Sigma Aldrich Fine Chemicals Biosciences CMV-CAS9-2A-GFP Plasmid | 1EA
CMV-CAS9-2A-GFP Plasmid | 1EA
HORIZON DISCOVERY BIOSCIENCES LIMITED EDIT-R LENTIVIRAL HEF1A BLAST
NC1763224 EDIT-R LENTIVIRAL HEF1A BLAST